Journal: Bioinformatics and Biology Insights

Article Title: Comparative Analysis of Sample Extraction and Library Construction for Shotgun Metagenomics

doi: 10.1177/1177932220915459

Figure Lengend Snippet: Experimental workflow. (A) Methods used for extraction of metagenomic DNA from human fecal samples. (B)-(C) Protocols used for metagenomic library preparation with different sample inputs.

Article Snippet: For DNA extraction, fecal and Zymo mock samples were performed using Mag-Bind® Universal Metagenomics Kit (Product# M5633-01, Omega Biotek) and DNeasy PowerSoil Kit (Catalog# 12888-100, Qiagen) ( and ) according to manufacturer’s instructions.

Techniques: Extraction

Journal: Frontiers in cellular and infection microbiology

Article Title: Porcine fungal mock community analyses: Implications for mycobiome investigations.

doi: 10.3389/fcimb.2023.928353

Figure Lengend Snippet: FIGURE 2 D24 fecal community. ITS1 (A), ITS2 (B), and 18S (C) primers were used to determine mean relative abundance of fungi found in post-weaning piglets (d24).

Article Snippet: Fecal and ATCC Reference Standard mock communities 6 piglet (d24) feces were sterilely collected from piglets (as above) and 250mg was used to prepare DNA for MiSeq to represent a typical fecal sample used in a microbiome study (Fecal Community).

Techniques:

Journal: Frontiers in cellular and infection microbiology

Article Title: Porcine fungal mock community analyses: Implications for mycobiome investigations.

doi: 10.3389/fcimb.2023.928353

Figure Lengend Snippet: FIGURE 6 (A) ATCC Reference Standard Sequencing Results ITS1. Technical replicates of the ATCC mock fungal community (ATCC Reference Standard 1 and ATCC Reference Standard 2) and DNA extracted from piglet feces (D24 feces) were sequenced on a MiSeq Illumina platform utilizing ITS1 primers. The UNITE database was utilized to identify taxonomy of the population. (B) ATCC Reference Standard Sequencing Results ITS2. Technical replicates of the ATCC mock fungal community (ATCC Reference Standard 1 and ATCC Reference Standard 2) and DNA extracted from piglet feces (D24 feces) were sequenced on a MiSeq Illumina platform utilizing ITS2 primers. The UNITE database was utilized to identify taxonomy of the population. (C). ATCC Reference Standard Sequencing Results 18S. Technical replicates of the ATCC mock fungal community (ATCC Reference Standard 1 and ATCC Reference Standard 2) and DNA extracted from piglet feces (D24 feces) were sequenced on a MiSeq Illumina platform utilizing 18S primers. The SILVA database was utilized to identify taxonomy of the population.

Article Snippet: Fecal and ATCC Reference Standard mock communities 6 piglet (d24) feces were sterilely collected from piglets (as above) and 250mg was used to prepare DNA for MiSeq to represent a typical fecal sample used in a microbiome study (Fecal Community).

Techniques: Sequencing